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Be Mindful of Pouch Placement: If you use Zyn pouches, try to rotate their placement to different areas of your mouth to minimize constant irritation to a single spot

Combining these strategies increases the chances of successfully reducing harm and eventually quitting smoking.

For detection of vsiRNAs, these were extracted using either Eurogold RNA pure (Euroclone) or the mirVana miRNA kit (Ambion) from freeze-dried mycelia (100 mg) collected at 10 dpi and ground to a fine powder with liquid nitrogen

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